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The MCS is maintained under negative pressure.
Such long-lived NUPTs might have been assigned new functions as genes or regulatory elements to be maintained under negative selection.
NHPs were exposed individually at a target dose of 1000 Plaque Forming Unit (PFU) of LASV Josiah in a head-only chamber in a class III biological safety cabinet maintained under negative pressure within a BSL-4 laboratory.
Similar(57)
Unexpectedly, even in the absence of the known editing site target, the truncated PPR genes are maintained under strong negative selection.
The coding sequences of the truncated CRR21 genes are maintained under strong negative selection; however, the 3' UTR sequences beyond the truncation site have substantially diverged.
We observed at least three independent C-terminal truncations of CRR21 genes in the Brassicaceae, yet these orphaned CRR21 genes are maintained under strong negative selection.
The truncated CRR21 genes are maintained under strong negative selection, and this suggests that they may be maintained for an unrecognized function that is potentially distinct from RNA editing.
PPR genes such as CRR4 and CLB19 that have evolved in the context of a homogenous requirement for editing are maintained under strong negative selection and gene architecture is stable.
Thus, both truncation of CRR21 and loss of the ndhD C383 editing site target have occurred in additional examples in angiosperm evolution, and these truncated CRR21 genes appear to be maintained under strong negative selection.
The retention of truncated forms of CRR21 that are maintained under strong negative selection even in the absence of an editing site target suggests that unrecognized function(s) might exist for this PPR protein.
Finally, analysis of CRR21 genes in sequenced genomes that have a genomic T at ndhD C383 demonstrates that CRR21 truncation is observed in three additional species of the Malpighiales, and that these genes are maintained under strong negative selection for what appears to be an extended period of evolution.
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