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hUCB-MSCs were maintained under hypoxic conditions (3% O2) with 1.8 mM Ca2+ during long-term culture, and their proliferation was evaluated.
In Figure 5A and 5B, the mean NAD(P H and FP concentration per cell is presented from hMSC cultures in either propagation, osteogenic or adipogenic medium maintained under hypoxic (5% oxygen) (HP, HO, HA) or normoxic (20% oxygen) (NP, NO, NA) conditions.
Oxygen homeostasis in normal articular cartilage is maintained under hypoxic conditions.
When maintained under hypoxic conditions, TH-VHLKO mice showed abnormally high hematocrit and EPO plasma levels on day 7 compared with wild-type mice (Fig 8E and F).
However, before cells were exposed to the hypoxic condition, they were treated under normoxic condition for 1 h then maintained under hypoxic conditions for 16 h.
A significant lowering in steady-state [Zn2+]cyt of 30 40% in preparations from either species maintained under hypoxic conditions (Fig. 1c, d) was detected.
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In this study, we applied TPEF and SHG imaging to monitor the progress of hMSC cultures stimulated with adipogenic and osteogenic differentiation factors and maintained under atmospheric or hypoxic (5%) oxygen concentrations.
For the first assay we maintained cells under hypoxic condition and for the second assay we placed cells in normoxic condition.
To test whether hypoxia could prevent myotube degeneration following acute inactivation of Rb plus its relatives, Rbf/f, Rbf/f:p130−/−, Rbf/f:p107−/− and Rbf/f:p130−/−:p107−/− myoblasts were transduced with Ad.cre or Ad.EV and then either maintained under normoxia or shifted to hypoxic conditions.
We therefore assessed the survivorship of hypoxic flies maintained under different feeding conditions.
Similarly, cardiomyocytes have been shown to maintain viability under hypoxic conditions ranging from 2 48 h in duration (Chandel et al, 1995; Budinger et al, 1996, 1998).
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