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After spraying, the cement tank was completely covered with the tent, and the inoculated seedlings were maintained under dark conditions for 24 h.
Fluorescent readings were taken immediately (time 0) and every 15 min over 60 min, with the plates maintained under dark conditions and incubated under exposure conditions (37°C/5% CO2) between measurements.
The plates were then maintained under dark conditions at 22°C for 18 h, at which time a volume of 5 μl of lactophenol cotton blue stain was added to each drop to dye the urediniospores and germ tubes that had been able to form.
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Mice were weaned between three and four weeks of age unless otherwise stated and then housed 3 4 per cage with food (PicoLab Mouse Diet 20, LabDiet) and water ad libitum, and maintained under 12∶12 light dark conditions.
Well-defined and reproducible diel patterns in cry1, cry2, and clock expression were observed in both field-collected and the experimental colonies maintained under control light:dark conditions, but no pattern was observed for cycle.
Inbred female BALB/c mice (6 8 weeks old, purchased from Iran Pasteur Institute) were maintained under 12-hour dark and light cycle, with free access to food and water.
Adult male cockroaches, Periplaneta americana, were obtained from a laboratory colony maintained under a light-dark cycle (LD 12 12) at 26 28°C.
Mice were housed in individual cages (5 for each group) and maintained under 12∶12 light-dark cycle at 21±1°C and 50±5% humidity.
Animals were maintained under a 12 12 light:dark cycle, with lights on at 08.00h and food and water were available ad libitum.
Animals were housed in standard cages and maintained under a 12h/12h light:dark cycle with food and water available ad libitum.
Athymic Balb/c male nude mice at 7 weeks of age were purchased from Dae-Han Biolink (Eumsung, Korea) and maintained under 12 h light: dark periods at 24 ± 2°C and were housed and handled under aseptic conditions.
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