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To this solution, 5 ml of 1 % tri-sodium citrate was added drop wise and the temperature was maintained under continuous stirring.
The organisms were maintained under continuous ventilation at 13°C ± 1°C and with a photoperiod of 12 12 h at 750 to 900 lux in artificial pond water (APW) (294 mg/L CaCl2 · 2H2O, 123.3 mg/L MgSO3 · 7H2O, 64.8 mg/L NaHCO3 and 5.8 mg/L KCl in deionised water) [[13]] prior to and throughout experimentation.
To measure the variations in the intracellular Ca2+ concentration ([Ca2+]i) induced by EGF, FGF, PDGF, UTP, ionomycin or thapsigargin, the samples were transferred to a thermostatted cuvette (37°C), maintained under continuous stirring, and analyzed in a Perkin Elmer LS-50B fluorimeter as described [15].
The birds were maintained under continuous lighting, and food and water were provided ad libitum.
The suspension was maintained under continuous stirring, overnight, at 4°C, and then filtered.
Hatching jars were maintained under continuous fluorescent light in a water bath at 24 ± 1°C and vigorously aerated to suspend embryos.
Similar(49)
Thus, the experiment was maintained under semi-continuous culture conditions.
Plasmids were maintained episomally under continuous antibiotic selection.
The in vitro plantlets were maintained under a constant temperature of 25 ± 2°C with continuous lighting of approximately 32.5 μmol m−2 s−1 light intensity.
Cultures were maintained under a light intensity of 50 µmol−2 s−1 at 25 °C and 24 h continuous light using white fluorescent light.
Following continuous breeding, Dnmt1+/− mice colonies and corresponding Dnmt1+/+ littermate control colonies were maintained under identical conditions.
More suggestions(14)
maintained under natural
maintained under standard
maintained under constant
maintained under clean
maintained under isothermal
maintained under local
maintained under specific
maintained under general
maintained under sterile
maintained under multirate
maintained under normal
maintained under static
maintained under magnetic
maintained under pathogen-free
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