In tissue engineering it is important that the scaffold allows cells to attach, spread, maintain their differentiation status or differentiate into functional cells in order to exhibit their physiological function.
Thus, the main aim of our study was to develop biocompatible and biodegradable MCs that can support high MSC yields while maintaining their differentiation capability and potency.
Moreover, cell polarity appeared well preserved on this substrate, so polarized cells like hepatoma HepaRG cells might maintain their differentiation status and act as primary human hepatocytes for hepatotoxicity testing.
Together with clonogenic culture conditions, FGF-2 supplementation prolongs the life span of bone marrow stromal cells to more than 70 doublings and maintains their differentiation potential until 50 doublings.
These results suggest that Ker-CT-Ras keratinocytes maintain their differentiation expression profile irrespective of their randomized location, but that subsequent modifications alter the cellular expression profile.
These hES cell derived hepatic progenitor cells could maintain their proliferation capacity for more than 100 days of culture in vitro, while maintaining their differentiation potential into both hepatocyte-like and cholangiocyte-like cells.
In addition it is now clear in the literature that growth of hMSCs at low oxygen tensions maintains their differentiation capacity[50], [58], which may be due to a reduction in oxidative stress induced aging [5].
Localized NKX3.1-mediated TNFα-JNK signaling in prostate epithelial cells may promote and maintain their differentiation state thus suppressing tumorigenesis.
