These were the conditions used to determine the KGDP for interaction of E. coli EF-Tu with GDP, and we maintained the same conditions in the determination of the KGTP.
At proper intervals, 2 mL of solution was taken for released protein measurement and returned back to the beaker to maintain the same condition throughout the experiment.
In the case of control, sterile distilled water was sprayed to maintain the same conditions as other treatments.
The necessary instructions are provided to ensure that during the scanning, while resting, the subject retains their usual dental occlusion (so as not to alter their anthropometric measurements), with the lips at rest and maintaining a very natural facial expression; even the acquisition while the subject is smiling has to maintain the same conditions.
In order to maintain the same conditions for all the slices used in the experiment, the tissue does not be immersed in the citraconic anhydride solution until the water bath reach the 95°C.
Thus, to maintain the same conditions of choice across doses (i.e., same delay between end of drug effects and next choice) and to avoid drug accumulation, the inter-trial interval was increased with the dose: 10 (4.3+5.7), 16.4 (10.7+5.7) and 23.1 (17.4+5.7) min for 0.25, 0.75 and 1.5 mg, respectively.
Videocapillaroscopy was performed in the morning to maintain the same conditions of the examination.
To maintain the same conditions for the collection of blood samples, patients and control participants were instructed to fast for 12 hours overnight while maintaining adequate hydration.
The secondary PCR was performed with the other set of primers for 20 cycles maintaining the same conditions using ten-fold diluted product of the primary PCR.
To address this point, parental BJAB, BJAB-Tet, and BJAB-Tet-WNT4 cells were cultured in the presence of Doxy for 48 h to maintain the same conditions for WNT4 expression, then, we analyzed total DNA content using flow cytometry to determine the size of cell populations in the different cell-cycle phases.
Control cell groups were treated with DMEM with 10% fetal bovine serum in the absence of CoCl2 and were maintained under the same condition.
