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As depicted in Figure 1D, NAFs reached full confluence after 7 days in culture and maintained the same cell number throughout the duration of the experiment.
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Cells were then resuspended in 2% glucose medium, maintaining the same cell concentration.
How these five structurally and functionally distinct sets of tubulin containing structures are constructed and maintained in the same cell is an intriguing problem.
Transfected CAL 27 cells (CAL 27-TF16) were maintained in the same cell culture conditions as described above for non-transfected CAL 27 cells.
In contrast, the control 10AT-Neo cell population maintained the same phenotype as the starting preneoplastic MCF-10AT cells with nearly undetectable to low levels of CD44, CD24, ALDH-1 and nucleostemin.
Jang et al. used magnetic nanoparticles (Fe3O4/SiO2) to control movement of human natural killer cells (NK-92MI) by an external magnetic field, loading NK-92MI cells infiltrated into the target tumor site and their killing activity is still maintained the same as the NK-92MI cells without the nanoparticles [ 99].
Thereafter, the cells maintained the same level of low but active growth to produce LA.
Both luciferases maintained the same spectrum of emission in cells at 37°C.
Starting from three days up to 1 week after seeding (Fig. 3 J L), the cultured cells maintained the same morphology, appeared closely adherent among themselves and were positive for May Grunwald Giemsa differential staining (Fig. 3 L).
The study of expression levels of TBCC, α tubulin and β tubulin in the tumors extracted from the mice revealed that the MC+ cells maintained the same alterations in vivo as those reported in vitro (data not shown).
All cells in other cell lines also maintained the same karyotypes as original chromosomal analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com