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Two extra introns had been predicted for the dog POMC gene, but none of the splice sites were optimal and the first intron maintained the reading frame.
Alternative splicing which maintained the reading frame (i.e. added or removed a multiple of 3 bases from the transcript) was clearly preferred for the 3' splice site (Figure 6).
Although an indel mutation in the ycf1 and ycf2 coding region was detected in Wogon-Sugi, sequence variations similar to that of Wogon-Sugi were also detected in several wild-type lines, and they maintained the reading frame.
Approximately 10% normal DYSF protein levels were expressed because the branch point mutation caused exon 32 skipping, which maintained the reading frame and produced shorter but functional protein, resulting in mild symptoms.
Sequence analysis identified two such deletions, a 15 bp deletion that maintained the reading frame, and a 103 bp deletion that induced a frameshift, truncating the predicted notch3 reading frame after the 16th EGF repeat (Fig. 1B,C).
Sub-cluster F, composed of a single OTU (i.e. OTU0015, which was identical to OTU002, but with a 54-nucleotide deletion that maintained the reading frame) was exclusively identified in one individual from PH.
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As a result we maintained the reads with a length between 25 nt and 70 nt.
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The loss of only exon 45 disrupts the reading frame, and hence it is necessary to excise either exons 44 + 45 or 45 + 46 to maintain the reading frame.
The deletion of exon 9 is known as a common splice variant maintaining the reading frame in dogs and humans [17], [18] (Figure 1A).
Also, the canine ITDs maintain the reading frame of the FLT3 protein.
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