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ES cells cultured on the E-cadherin-expressing feeder cells maintained the expression of stem cell markers, alkaline phosphatase (AP), Oct3/4, Nanog and Sox2, and the efficiency of AP-positive colony formation was comparable to MEFs, and much better than parental STO and NIH3T3 cells.
More importantly, neurons in the adult male substantia nigra were shown to express Sry, which maintained the expression of tyrosine hydroxylase, the rate limiting enzyme of catecholamine (dopamine) synthesis [ 17].
The induced clusters lost their expression of the cholangiocyte marker KRT7 while maintained the expression of AFP and began to express ALB (Figure 5B and C), which was only faintly expressed in hepatic progenitor cells.
In contrast, the combined treatment with HTNCre and FTC showed that p53-shRNA-expressing cells (GFP-negative cells, arrows in Fig. 4E) maintained the expression of Nanog that is higher compared to adjacent GFP-positive cells, which did not express p53-shRNA (arrow heads in Fig. 6E).
Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100.
Sphere-forming cells in group CL, however, maintained the expression of these undifferentiated markers.
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Our previous studies have shown that the MKK1/2-ERK1/2 MKK1/2-ERK1/2y maintainsignalexpathwayn of Rad51 in NSCLC cells.
This indicates that compensation by the rhl QS system could override this hierarchy and maintain the expression of QS-dependent virulence factors in spite of a non-functional central las system.
Monocytes cultured with GM-CSF and IL-4 for 4 days exhibit markers characteristic of iDC and maintain the expression of both formyl peptide receptors FPR and FPRL1, as well as CCR5.
The results showed that elongated morphology (Group C) could help in vitro passaged tenocytes to retain their phenotype and function by maintaining the expression of tenomodulin (tenocyte marker) and collagen I (functional molecule).
Thus, LRP5 is important for stimulating or maintaining the expression of Slc38a5 gene in Müller cells.
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