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AdMSCs within the 3-D aggregates maintained the cell surface epitopes of AdMSCs with high viability.
Notably, the miniaturization and introduction of monolithic structure in the anode support maintained the cell dimension within the 'micro-tubular' range, which was essential for a high volumetric power density.
This formulation maintained the cell survival rate to greater than 90% and the activity of Enterobacter sp. NP23 to Scenedesmus sp. did not show apparent decrease even after 18 months of storage.
The compressive modulus and degradation properties of collagen hydrogels were ameliorated after cross-linked, and chondrocytes encapsulated in the cross-linked hydrogels proliferated well and maintained the cell phenotype.
We then maintained the cell culture at 37 °C, a temperature best supporting the growth of epithelial cells.
NK maintained the cell cultures and provided technical help.
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However, these authors used a much higher DMSO concentration and maintained the cells at 10.5 °C, which is obviously different to our experimental setup (37 °C).
Moreover, it was not determined whether the surfaces had maintained the cells phenotype.
We maintained the cells in Dulbecco's modified Eagle's medium (Invitrogen, Carlsbad, CA, USA).
Next, we maintained the cells in phenol red-free DMEM with 0.2% charcoal-stripped FBS for 24 h.
Most importantly, the microspheres can maintain the cell type.
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