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The means by which sublimons are maintained, propagated, and transmitted largely remain unexplored.
All cell lines were maintained, propagated, hormone-starved for 5 days and analyzed for estrogen signaling as described earlier [ 21, 45].
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Plasmids were maintained and propagated in E. coli DH5α.
In a blog post from October 2016, former leader Damigo describes Project Siege as the "beginning of a long term cultural war of attrition against academia's Cultural Marxist narrative that is maintained and propagated into society through the indoctrination of the future managerial class".
Cell cultures were maintained and propagated following supplier's protocol.
Only cells that attached to the culture flask were maintained and propagated by passage.
Recombinant plasmids were then maintained and propagated in DH5α E. coli.
The resistant cells designated as H2B-TxR were maintained and propagated in the presence of 5 nM Taxol.
HEK293 cells were maintained and propagated in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and 2 mM glutamine in a humidified 95% air, 5% CO2 incubator at 37°C.
RG cells are also fated to become glial cells in the post natal brain and a subset of them is maintained and propagated in specialized germinal/neurogenic niches of the adult brain, as neural stem cells (NSCs) [1].
Generally, since some Sebacinales can easily be maintained and propagated without their plant hosts, these strains may be ideal models for the study of beneficial fungus-plant interactions and have a promising perspective for application in sustainable horticulture and agriculture [15], [17], [18], [21], [22].
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