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The stain for osteocalcin was used and showed that cells maintained phenotypic expression for this bone specific protein.
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Both LSECs and KCs maintained phenotypic markers.
Despite the scientific and technical hurdles that must be overcome, substantial progress has been made in recent years and the newer hepatic culture technologies have begun to incorporate more of the specific features that restore and maintain phenotypic architecture and gene expression profiles in vivo.
How cells maintain phenotypic states in the face of environmental fluctuations remains an open question.
The evolution of condition-dependent expression of ornamentation may maintain phenotypic and genetic variability in sexually selected traits as condition itself is expected to have high genetic variance.
Should TFEB expression fade over time, to maintain phenotypic correction it would be possible to re-administer a vector with a different serotype to overcome the neutralizing anti-Ad antibody elicited with the first administration (Kim et al, 2001; Morral et al, 1999).
In adult organisms, the CTCF protein is ubiquitously expressed across most tissues, and its expression levels vary in a cell type-specific manner, suggesting a role in maintaining phenotypic diversity [ 4].
NIH 3T3 fibroblast cell seeded on the structure tend to maintain phenotypic shape and guided growth according to nanofiber orientation.
To date there are very few phenotypic selection studies of ecotypes showing that contemporary selection maintains phenotypic differences between populations.
Therefore, culture systems are needed that maintain phenotypic function for weeks or even months.
Each of the printed cell types maintained their viability and normal proliferation rates, phenotypic expression, and physiological functions within the heterogeneous constructs.
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