Sentence examples for maintained overnight from inspiring English sources

Human umbilical vein endothelial cells were cultivated at 37 °C in 48-well plates in RPMI 1640 medium supplemented with 10% fetal calf serum (FCS) and maintained overnight in a humidified incubator (5% CO2).

These conditions were maintained overnight to allow the fish to acclimate.

All cultures were maintained overnight then Alamar Blue (Biosource International) was added to a final concentration of 10% (v/v).

Cells were maintained overnight and the next day, switched to a differentiation medium consisting of growth medium with reduced FBS (2% final) and 8 µM retinoic acid (Sigma, St Louis, MO).

Hypothalami from rats fed on control and HF diets were dissected and maintained overnight at 4°C in fixative containing 2.5% glutaraldehyde and 0.5% paraformaldehyde in phosphate buffer (pH 7.4).

IPTG was added to 0.5 mM and protein expression was maintained overnight at 20°C.

The airway management was possible by maintaining overnight the intubation in the other patients.

Each participant received either 50-mg oral naltrexone or identical placebo during the morning of the session after maintaining overnight abstinence.

The Senate bill proposes cutting about half the mail processing centers the Postal Services wants to close, from 252 to 125, and allowing more areas to maintain overnight first-class mail delivery for at least three more years.

Divalproex sodium coated particles (Depakote® sprinkle capsules, 125 mg per capsule) were administered in divided doses two to three times daily sufficient to maintain overnight trough levels of 50 100 mg/dL.

She has maintained an overnight vigil at the hospital.