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We find that adult medfly and D. melanogaster have opposite responses to the absence of yeast: D. melanogaster have high mortality when on no-yeast diet; when switched to full diet, D. melanogaster reduce mortality rates to the level presented by females continuously maintained on yeast.
Candida albicans strain SC5314/ATCC® MYA-2876™ (American Type Culture Collection, Manassas, VA, USA) was maintained on yeast extract-peptone-dextrose (YPD) medium (1% w/v yeast extract, 2% w/v glucose, and 2% w/v peptone) and incubated at 30°C.
Filamentous fungi were maintained on malt extract agar slants (MEA: 0.5 % malt extract, 0.25 % yeast extract, 1 % glucose, 2%% agar (w/v)), yeasts were maintained on yeast extract glucose medium (YEGK: 1 % glucose; 1 % KH2PO4; 0.5 % yeast extract, 2%% agar (w/v)) at 4 °C.
H99 was maintained on yeast extract peptone dextrose (YPD; 1% yeast extract, 2% peptone and 2% dextrose) agar plates at 30°C.
Each strain was maintained on Yeast Peptone Dextrose (YPD) agar (2% glucose, 2% peptone, 1% yeast extract, 2% agar) at 30°C and then subjected to the studies described below.
Fungal cultures were maintained on yeast nitrogen base (YNB) medium containing 0.67% (w/v) YNB Difco LaboratoriesSparksrks, MD) and 1% (w/v) glucose in the presence or absence of nematode cuticle.
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The strains were maintained on yeast-extract-peptone-dextrose (YPD) media (1% yeast extract, 2% peptone, 2% dextrose, and 2% Bacto agar).
Both strains were routinely maintained on yeast-peptone-dextrose (YPD) medium containing (w/v): 2% glucose, 2% Bacto Peptone and 1% yeast extract - including 2% agar when required (all media components supplied by Difco).
Bradyrhizobium japonicum USDA 110 and ZB977 were maintained on the yeast extract-mannitol agar (YMA) with chloramphenicol (30 µg/mL) and tetracycline (50 µg/mL), respectively, and cultured in yeast extract-mannitol broth (YMB) without antibiotics for the induction experiments in the presence of SSE or genistein.
The strains were grown and maintained on rich yeast extract-Peptone-Dextrose (YPD) media (2% (w/v), glucose; 2% (w/v) bactopeptone; and 1% (w/v), yeast extract) or YPD agar solid media (YPD with 2% agar).
All fly stocks were maintained on standard yeast protein, carbohydrate, agar media at the indicated temperatures (18°C or 26°C) according to standard procedures.
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