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The strain was maintained on solid medium at 4 °C.
The fungi were maintained on solid agar plates.
Stock cultures were maintained on solid agar slants at −120°C.
Legionella pneumophila bacteria were maintained on solid agar plates BCYE (buffered charcoal yeast extract).
Stocks of yeast strains were maintained on solid 2% YPD (1% yeast extract, 2% glucose, 2% peptone and 2% agar) in proper conditions to avoid the selection of petites or suppressors.
When the tlc1 mutant was maintained on solid plates, cells displayed sensitivity to bleomycin from the first streak, and they showed much lower viability on bleomycin plates for the following restreaks (Figure 3A, top), even after Y' amplification was generated at the 3rd restreak, as it was evident by Southern blot analysis (Figure 3B, top).
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To replicate the findings of previously published experiments by Petrascheck and colleagues [24], we have we have applied both compounds described to be life-extending in the original paper, mianserin and methiothepin, to Bristol N2 C.elegans which in our case were maintained on solid-phase agar media, as described in Material and Methods.
Debaryomyces nepalensis NCYC 3413 was maintained on a solid YEPP medium containing yeast extract 10 g/l, peptone 20 g/l and pectin 5 g/l at pH 7.0 and incubated at 30°C for 26 h and later stored at 4°C.
When we attempted to validate this phenotype, we found that the cells would partially recover normal signal over a period of several days, if maintained on a solid growth medium (Figure S2A).
The fungi were maintained on a solid medium containing 20 g L−1 malt extract and 15 g L−1 Agar-Agar.They are deposited in the culture collection of IMD Natural Solutions GbR (formerly InterMed Discovery GmbH under liquid nitrogen.
All strains were maintained on semi-solid CYM medium (Yamagishi et al. 2007) at 28 °C.
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maintained on endocrine
maintained on 12-hr
maintained on nutrient
maintained on continuous
maintained on 3-cm
maintained on secure
maintained on mixed
maintained on standard
maintained on conventional
maintained on regular
maintained on irradiated
maintained on low
maintained on normal
maintained on oral
maintained on dry
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