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This strain can be maintained on nutrient broth (or L broth) only if the medium is supplemented with 40-50 ug/ml of DL-diaminopimelic acid.
All strains were maintained on nutrient agar.
All bacterial pathogenic strains were maintained on nutrient agar slants.
Cultures were maintained on nutrient broth at 37 °C.
Pseudomonas putida KT2440 (ATCC 47054) was maintained on nutrient agar plates at 4°C.
Different bacterial strains maintained on nutrient agar and subcultures were freshly prepared before use.
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For example, we might expect a striking decrease in reproductive output or survival, which was not observed for Spiroplasma-infected flies maintained on a nutrient poor diet.
The culture was maintained on the nutrient agar medium (w/v) (beef extract 0.3%, peptone 0.5%, sodium chloride 0.8%, and agar 1.5%) and stored at 4°C.
Importantly, we observed that Spiroplasma proliferation in whole flies and hemolymph was also significantly inhibited when flies are maintained on a nutrient poor diet.
Quantification by qPCR reveals that Spiroplasma titers are significantly lower in the hemolymph of flies maintained on a nutrient poor diet for 12 days than flies maintained on a rich diet.
After 4-week treatment with AC3174, a GLP-1 receptor agonist, DIO mice had a mean 2-h fasting plasma glucose of 170 ± 4 mg/dl, which was similar to that of mice maintained on a nutrient matched LF diet and 15% less than the mean 2-h fasting plasma glucose of 205 ± 8 mg/dl in the vehicle-treated mice (p < 0.01).
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maintained on endocrine
maintained on 12-hr
maintained on continuous
maintained on 3-cm
maintained on secure
maintained on mixed
maintained on standard
maintained on conventional
maintained on oral
maintained on normal
maintained on dry
maintained on irradiated
maintained on artificial
maintained on regular
maintained on low
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