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Group II male rats were maintained on normal diet mixed with corn oil as oil control.
or 25 ppm chromium or were dosed with a combination of ethanol+chromium at the same concentrations for a period of 22 weeks ad libitum and were maintained on normal diet.
Group II female rats were maintained on normal diet mixed with corn oil at 16.5 mg/kg diet/day and 0.94 mg/kg body weight/day as oil control.
Prior to analysis flies were maintained on normal diet or fasting diet for 48 hours.
After two generations on tetracycline-containing food, flies were subsequently maintained on normal food to recover for at least five generations.
After the first locomotor activity quantification, flies were re-collected and maintained on normal food medium during 3 additional days, after which locomotor activity was again recorded and the start/stop numbers determined.
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Methods: Saphenous veins were placed as bilateral interposition grafts in femoral arteries of two groups of adult male mongrel dogs; one group was maintained on a normal diet (control), the other group supplemented with L -arginine (200 mg/kg per day) beginning 1 week before surgery.
Control animals were maintained on a normal diet and water for the same period.
The animals were maintained on a normal 12 h light and dark cycle, at a temperature of 25 27 °C, relative humidity of 10 ± 60%% and were allowed 7 days to acclimatize prior to study commencement.
Animals were divided into: rats maintained on a normal diet (control group); rats maintained on a 5% high-cholesterol diet for 8 weeks (HC group); and rats maintained on a 5% high-cholesterol diet for 8 weeks and administered resveratrol (20 mg/kg) orally for 2 weeks (HCR group).
This observation was confirmed by another group for an independently generated Atxn2 knockout line maintained on a "normal" diet [15].
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