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The wild-type C. elegans Bristol strain N2 was obtained from Caenorhabditis Genetic Centre (CGC), USA, and culture was maintained on nematode growth medium (NGM) plates seeded with Escherichia coli strain OP50 at 20 °C, using the standard method [22].
Animals were maintained on nematode growth media (NGM) plates seeded with Escherichia coli OP50.
Animals were maintained on nematode growth medium seeded with Escherichia coli OP50 (Brenner 1974).
Animals were maintained on nematode growth media (NGM) with OP50 E. coli at 20°C (Brenner, 1974).
All strains were routinely maintained on nematode growth medium (NGM) with E. coli OP50 as a food source [ 69].
Worms were maintained on nematode growth medium (NGM) agar plates, fed the OP50 strain of Escherichia coli, and grown at 20.0°.
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All strains were maintained on solid nematode growth medium (NGM) overlaid with E. coli (OP50), at 20 °C except for upshift of strains CL4176 and CL2355 to 25 °C during the L3 L4 transition to induce Aβ1 42.
All strains were maintained on E. coli OP50-seeded Nematode Growth Medium (NGM) plates as previously described [ 16].
N2 nematodes were developed on one bacterial strain and late L4 stage nematodes were subsequently maintained on the same strain or transferred to a different one.
Nematodes were maintained on NGM plates at 20 °C with the E. coli strain OP50 as a food source [ 69].
However, it was reported that daf-16 and dbl-1 mutants have an increased amount of live bacteria in their intestine compared to N2 nematodes when maintained on E. coli OP50 [ 49].
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