Sentence examples for maintained on mouse from inspiring English sources
Most prior established iPS cell lines were derived and maintained on mouse embryonic fibroblast (MEF) cells supplemented with exogenous leukemia inhibitory factor (LIF).
Mouse ES cells were maintained on mouse MEF feeder cells in the presence of leukemia inhibitory factor (LIF) in DMEM containing 15% Knockout™ Serum replacement (Invitrogen).
Human pluripotent stem cells, both embryonic stem (ES) cells and induced Pluripotent Stem (iPS) cells, are generally maintained on mouse embryonic fibroblasts (MEF), which are mitotically inactivated by treatment with mitomycin C or γ-ray irradiation [1] [3].
R1 [68] and E14TG2a [69] mESC lines were maintained on mouse embryonic fibroblasts (MEFs) inactivated with 10 µg/ml mitomycin C (Sigma) in ESC medium (DMEM supplemented with 15% FBS {Hyclone}, 1% penicillin/streptomycin {Gibco}, 0.1 mM 2-mercaptoethanol {Gibco}, and 0.1 mM non-essential amino acids {Gibco}) with 1000 U/ml LIF (Chemicon).
Colonized mosquitoes were maintained on mouse blood (for egg laying) and given 10% sucrose ad libitum.
Since mink pluripotent cells were maintained on mouse feeder cells raw reads produced by next generation sequencing might contain transcripts of mouse feeder origin.
Wild-type C57BL/6 mice, maintained on standard mouse chow and normal drinking water, were used in parallel as controls.
All mice were maintained on PicoLab mouse diet 20 with soybean as a main protein source.
All mice used in this study were maintained on regular mouse diet (6% fat IsoPro 3000 from Purina that contains 6% fat).The mice were fasted for 12 h, beginning one hour after the start of their light cycle.
hESCs were maintained on irradiated mouse embryonic feeder cells derived from C57BL/6 mice (E12.5 E13.5) in knockout-DMEM media as described [4] (Invitrogen).
This also eliminates the problems with strain background differences as mouse lines obtained from different laboratories might be maintained on different mouse backgrounds.
