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Heterogeneous populations of stable transfectants were selected in media containing 0.5 µg/mL of puromycin and maintained on media containing 0.25 µg/mL of puromycin.

To clarify the role of dfoxO during fasting, we studied the survival time of various homozygous null dfoxO flies maintained on media without yeast and sugar.

All Drosophila stocks were maintained on media consisting of corn meal, sugar, yeast, and agar in incubators maintained at a constant temperature of 21°C, or in a room that typically fluctuated between 21°C and 22.5°C.

Thereafter, clones were maintained on media containing 300 μg/ml G-418.

Stock BY-2 calli were maintained on media solidified with 0.7% (w/v) agar and subcultured monthly.

M. paratuberculosis is usually maintained on media containing ferric ammonium citrate rather than pyruvate, as the two are antagonistic once mycobactin is added [ 45].

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For the plasmid shuffle, strains were then plated on −Leu +Ura +5-FOA media and individual colonies were picked and thereafter maintained on −Leu media.

Cells were maintained on DMEM media and relative number of metabolically active cells was determined by MTT assay up to day six of culture.

Since GLUT5 conferred a rather slow growth on synthetic media (about five days until colonies appeared on agar plates; see Fig. 1a), we generated plasmids with dominant markers that can be maintained on rich media (YEP) for further functional studies.

Drosophila cultures were maintained on standard media.

Flies were maintained on standard media at 25°C.

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