For each strain, 0.5 ml aliquots of resuspended cells were transferred to two microfuge tubes: one tube was maintained on ice (control) and the other was incubated in a water bath at 52°C for 20 min followed by incubation on ice.
The samples were maintained on ice during transportation until processing.
Spermatozoa maintained on ice were equally viable (>95% for T300 and T600) for up to 80 min, whereas sperm viability in artificial fresh water (FW) at 27 mOsm/kg decreased (P < 0.05) to 67% after 5 min, with only 3.5% viability at 25 min.
All of the protein preparation was maintained on ice.
Cell suspensions were maintained on ice during transplantation procedures.
BM cell suspensions were maintained on ice throughout the purification procedures.
Inocula were maintained on ice and used within 1 2 h.
Just prior to analysis, frozen sera were thawed and maintained on ice throughout the assay setup.
The suspension was maintained on ice for 20 min, and the cells were then disrupted by pipetting.
Human serum from at least two individuals was heat inactivated (HIS) at 55°C for 45 min or was maintained on ice (normal serum, NS).
Cells were diluted to achieve a final concentration for injection of 30000 cells in 20 µl and maintained on ice prior to surgery.
