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For example, heterozygotes can simply be maintained on growth media containing G418 or identified under the fluorescent microscope by pharyngeal GFP expression.
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The wild-type C. elegans Bristol strain N2 was obtained from Caenorhabditis Genetic Centre (CGC), USA, and culture was maintained on nematode growth medium (NGM) plates seeded with Escherichia coli strain OP50 at 20 °C, using the standard method [22].
For cardiac mesoderm differentiation, hESCs maintained on Matrigel (growth factor reduced, BD Biosciences) in E8 were dissociated into single cells with Accutase (Invitrogen), then seeded onto Matrigel-coated tissue culture dishes at a density of 5 × 104 cells/cm2 and cultured in E8 for 3 days.
Animals were maintained on nematode growth media (NGM) plates seeded with Escherichia coli OP50.
Animals were maintained on nematode growth medium seeded with Escherichia coli OP50 (Brenner 1974).
Animals were maintained on nematode growth media (NGM) with OP50 E. coli at 20°C (Brenner, 1974).
All strains were routinely maintained on nematode growth medium (NGM) with E. coli OP50 as a food source [ 69].
hiPSCs were maintained on Matrigel (growth factor reduced; Corning 354230) in mTeSR1 medium (Stem Cell Technologies) and passaged with 1 mg/ml Dispase (Life Technologies).
Worms were maintained on nematode growth medium (NGM) agar plates, fed the OP50 strain of Escherichia coli, and grown at 20.0°.
All strains in this study were derived in the Bristol N2 background and maintained on nematode growth medium (NGM) plates seeded with OP50 (Brenner 1974).
Worms were maintained on nematode growth medium (NGM) plates seeded with Escherichia coli (OP50) at 15°C, using standard techniques (Brenner, 1974).
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