Sentence examples for maintained on feeder from inspiring English sources

ESCs and iPSCs were maintained on feeder cells, so condition medium collected from feeder cells only was used as the control group.

IPS cells were maintained on feeder layers of mitomycin C (10 µg/ml media, Sigma-Aldrich -treated REF cellSigma-Aldrich -treated

Sheep iPSCs were passaged by enzymatic dissociation using 1 mg/ml collagenase IV(Gibco and maintained on feeder layers of mouse embryonic fibroblasts (MEF) that were mitotically inactivated by mitomycin C (Sigma).

It will be of great interest to know if rat ESCs can be stably maintained on REFs. Given that both mouse and rat iPS cells are shown to be stably maintained on feeder cells originated from the same species, as shown in mouse [2] and rat iPS cells (this study), it will be interesting to learn whether human iPS cells can also be maintained on appropriate human feeder cells.

H9, HUES9, and HUES1 hESC lines were maintained on feeder layers of mitotically inactivated MEFs (2 × 10/cm; Millipore).

Briefly, undifferentiated hESC were maintained on feeder cell layers, which were mitomycin C treated mouse embryonic fibroblasts (MEF).

iPSCs were manually passed and maintained on feeder-free condition until the beginning of the differentiation protocol.

Mouse ESCs (R1 and R1/E) were maintained on feeders in standard medium.

Colonies resembling pre-iPSCs emerged at day 5 postinfection and could be maintained on feeders without reactivating Nanog GFP.

It has recently been reported that EpiSCs maintained on feeders in the presence of serum-derived components may produce ESC-like cells (Bao et al., 2009; Greber et al., 2010).

B6 ESCs were routinely maintained on feeders in DMEM medium (Gibco) containing 10% FBS (HyClone), 1 mM Sodium Pyruvate (Gibco), 100 µM Non-Essential Amino Acids (Gibco), 2 mM GLUTAMAX (Gibco), 1 µM β-mercaptoethanol (a formulation hereinafter referred to as "mESC medium"), and 1000 U/ml LIF (Stemgent).