Beetles were maintained on a diet supplemented with 2-mm cubes of fresh apple.
Stocks were maintained on standard Bloomington medium at 25°C, and embryos were collected at 25°C on apple juice agar plates.
Apples and water were provided ad libitum and the animal room was maintained on a 14L 10D cycle with ambient temperature (22°C).
Subjects were maintained on a diet of Nektar-Plus hummingbird food (Nekton Produkte, Pforzheim, Germany), cantaloupe, and a mixture of chopped banana, apple, fig, papaya, and marmoset chow, and had ad libitum access to water.
At this location, sheep were maintained continuously on the same meadow, from which we collected apples and where they most likely ate fruits, possibly limiting the time during which rotting apples were available as substrate.
Other apple microarrays were reported on apple tree scions [ 20] and fruitlet abscission [ 21].
Embryos were collected on apple juice-agar plates.
Embryos were collected on apple juice plates at 25°.
