Furthermore, mineral aggregates resist compression and shrinkage, thereby maintaining medium structure and further promoting drainage and aeration (Dunnett and Kingsbury, 2004).
Based on the consumption rates and corresponding concentration optimization, glucose and fast metabolized amino acids were supplemented into the maintaining medium at the time of infection.
The maintaining medium was serum-free DMEM (high glucose) with N2 supplement.
After 7 days in vitro slices were grown in maintaining medium (as above) without B-27 supplement.
For antigen testing, the culture medium of confluent cells were changed into fresh maintaining medium.
Twelve hours later, medium was changed with stem cell maintaining medium and kept in culture.
The fetal bovine concentration of the maintain medium (MM) was reduced to 1%%.
It was used swollen cellulose to prepare the strain maintaining medium.
For antigen amounts detection, in next day, the cells started growing in confluence and the medium containing 10% FBS were changed into maintaining medium (containing 1% FBS).
