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Four rats from each experimental group were maintained individually in metabolic cages for 15 days after surgery, and daily urine specimens were frozen for the urine sample analysis.
To investigate this we injected 30 fed, mated females in groups of 10 with mean weights of 0.6 g with PBS, rifampin, or tetracycline on day 0. The ticks were maintained individually in an incubation chamber and observed daily for oviposition.
Pupae were collected daily and females were maintained individually in grid boxes.
For survival assays, the embryos were similarly microinjected and maintained individually in 24-well plates in E3 medium at 28 °C.
The turtles were transported to the Instituto Nacional de Pesquisas da Amazônia in Manaus (Brazil), and maintained individually in plastic aquaria.
From the collected lice, a single female and male couple were placed onto one salmon, each maintained individually in single-fish tanks, in order to ensure single paternity.
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Chameleons were maintained individually or in small groups in either mesh-sided enclosures or glass terraria with live plants, depending on the species.
And in an aside that may worry the Russell Group of universities he did not rule out maintaining individually excellent departments even in a university which is not itself deemed among the elite.
In many forest guenons, including Campbell's monkeys, females form the social core of the group and maintain individually differentiated long-term bonds with one another [ 22].
Five to seven-weeks-old male athymic nude mice (Shanghai Laboratory Animal Center, CAS) were maintained in individually ventilated caging systems in groups of five at 19 23 °C, with a 12-h light-dark cycle.
Rats were maintained in individually ventilated cages (4 per cage) in the Animal Holding Facility (AHF) at the International Medical University (IMU) after their arrival.
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