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We found that the unknown factors in tested human plasma YN02 and YN06 stimulated the growth of host cells and resulted in overestimate of HIV-1 neutralization when the tested plasma were maintained in whole assay (Fig. 2).
When tested plasma was maintained in whole assay, the plasma at dilution of 1 4 (such as YN02 and YN06) stimulated cell growth, their protection rates distinctly were beyond 100% (Fig. 3).
Accordingly, cells were incubated for 1 h with 5 × 10−8 M ZnPc-DPPC solution, 10−6 M TMPyP or 5 × 10−8 M ZnPc+10−6 M TMPyP in PBS, washed three times with PBS and maintained in whole medium during irradiation and post-treatment time (1, 3, 6, 18, 24, or 48 h).
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Structural characterization of the chemically delithiated LixNi0.33Mn0.33Co0.33O2 samples indicates that the initial rhombohedral symmetry (α-NaFeO2 type structure) is maintained in the whole 0.3 ≤ x ≤ 1.0 composition range.
The conductance of the intrinsic graphene sheet is twice of the conductance quantum maintained in the whole voltage range as expected [1].
The genome may be more tolerant of mutation in the context of tumorigenesis, in which homeostasis need not be maintained in the whole organism, than in the evolution of species, in which this homeostatic constraint must be obeyed.
Previously, tested human plasma or sera were maintained in the whole cell culture of microtiter neutralization assay.
MCF-7 cells were grown in phenol free EMEM/CD-FBS media for 4 days prior to drug treatments and MCF-7/shRNA ERRα RNAi cells were maintained in normal media containing whole serum (described above).
Observation revealed that associations between HCEs and target genes were maintained in both copies after the whole genome duplication in teleosts with the loss of bystander genes, and that "genomic regulatory blocks" (GRBs) correspond to the long regions of conserved gene order across vertebrate genomes [19].
Optimal SAS and PI scale scores were well maintained in the group as a whole.
A culture of participation should be developed and maintained in the units and the whole hospital to ensure that all staff feel they play important roles in the hospital.
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