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A verification of residual load-bearing capacity is necessary to determine if the structure can be maintained in use.
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In contrast, some studies use B16F10 transfected with chicken ovalbumin, a foreign antigen that is sometimes maintained in cells using the neo gene for G418 selection which is itself a strong antigen for immune rejection [41].
Stock culture of malaria parasite Plasmodium falciparum 3D7 strain was continuously maintained in vitro using the candle-jar method [30].
hBMSCs were seeded in a scaffold (collagen sponge) that offers a high level of complexity, recapitulating the 3D organization of chondral tissue, and maintained in culture using the highly chondrogenic TGF-β1 growth factor.
T. evansi camel isolate was maintained in vivo using Swiss albino mice through serial passage in the laboratory.
Eggs from each cross were maintained in isolation using standard commercial rearing methodologies.
Total RNA was isolated from LNCaP cells maintained in vivo using TRIZOL® Reagent according to the manufacturer's protocol.
Stable pooled populations of FRNK-MMP-2 cells were maintained in culture using 20 μg ml−1 of G418 or puromycin.
However, a selective set of metabolism pathways is maintained in chloroplasts using both chloroplast genome encoded and nuclear genome encoded enzymes.
Since SOX2 is associated with gastric differentiation [ 14] we studied whether this was maintained in cancer, using MUC5AC as a gastric marker.
A fixed concentration of the product 1 (0.152 mg mL-1) was maintained in all samples used to construct the calibration curve.
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