The following exposures were maintained in triplicate: (1) abiotic control; (2) FeOB Mariprofundus sp. M34; (3) FeRB S. japonica; and (4) a combination of FeOB Mariprofundus sp. M4 and FeRB S. japonica.
To generate growth curves, both irradiated and unirradiated HMEC cultures were maintained in triplicate through Passage 9. Low linear energy transfer (LET) exposures were conducted using either 160 kVp X-ray or Cs γ-irradiation.
The control and APP-kd of MDA-MB-231cells (2×10) were seeded in 6-well plate in triplicate and maintained in normal growth medium.
In the colony formation assay, 3-5 × 10 of the Hep2 cells at twelve hours after transfection were seeded in a 60-mm Petri dish in triplicate and maintained in RPMI 1640 (GIBCO, Los Angeles, CA) with 10% fetal bovine serum.
Twenty-four hours after transfection, 2000 transfected HepG2 and Huh7cells were placed in a fresh six-well plate in triplicate and maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum for 2 weeks.
Twenty-four hours after transfection, 1000 transfected HepG2 and Huh7 cells were placed in a fresh 96-well plate in triplicate and maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum for 5 days.
For growth curves, cells were plated on day 0, maintained in 10% FBS and triplicates were counted every 24 h.
MCF-7 cells were maintained in phenol free EMEM/CD-FBS media for 4 days prior to drug treatments (in triplicate) and MCF-7/shRNA ERRα RNAi cells were maintained in normal media containing whole serum (described above).
Triplicate cultures were initiated from an OD600 of 0.05 and maintained in four different conditions: 37°C with and without IPTG induction, and 20°C with and without IPTG induction.
