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Virgin female Wistar-Furth rats obtained from Harlan Sprague Dawley, Inc., (Indianapolis, IN) were maintained in temperature- and light-controlled (14 h light, 10 h dark cycle) conditions in the Tufts University School of Medicine Division of Laboratory Animal Medicine.
All of the animals were maintained in temperature (23 ± 1°C -controlled and humidity (50% ± 5%)-controlled rooms under a 1°C -controlled cycle and provided water and food ad libitum.
CD-1 female mice (12 weeks of age) and proven breeder male mice (all from Charles River Laboratories, Wilmington, MA, USA) were maintained in temperature- and light-controlled (14/10-hr 14/10-hrrk cyclight/darkions at the at Tufts University Human Nutrition and Researcyclenter animal faconditionsproved by the atsociathen for atsessmenTufts University Human Nutrition and Research Centeranimall).
Sexually mature virgin female Sprague-Dawley rats (8 10 weeks of age; Taconic, Germantown, NY) were maintained in temperature- and light-controlled (14 hr/10 hr light/dark) conditions in the Tufts University School of Medicine Division of Laboratory Animal Medicine.
Twelve-week-old female CD-1 mice and proven breeder male mice (Charles River Laboratories, Wilmington, MA, USA) were maintained in temperature- and light-controlled (14 hr/10 hr light/dark cycle) conditions at the Human Nutrition and Research Center animal facility (Tufts University), a facility approved by the Association for Assessment and Accreditation of Laboratory Animal Care.
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BALB/c mice (NCLAS, Hyderabad, India) weighing 20 25 g were maintained in temperature-controlled room with light-dark cycle.
Swiss albino mice (NCLAS, Hyderabad, India) weighing 20-25 g were maintained in temperature-controlled room with light dark cycle.
All mice were maintained in temperature-controlled conditions (20 22 °C) under 12-h light/12-h dark cycles.
Metabolites were separated on a Diamond Hydride column (4 μm, 100A 2.1 × 150 mm, MicroSolv Technology, Eatontown, NJ, USA), which was maintained in temperature-controlled chamber (37 °C).
Animals were maintained in temperature-controlled room (22±2°C) with a 12 h light/dark cycles and free access to food and water.
Our flies are maintained in constant temperature, so the light signal is not associated with increased temperatures as it would be in the wild.
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