Pancreatic adenocarcinoma cell lines were grown in vitro and maintained in standard culture medium.
The Wik wild-type strain of zebrafish was used for this study, and they were maintained in standard conditions59.
Ovariectomized young and aged female mice were maintained in standard or enriched housing for 4 weeks prior to and then throughout object recognition testing.
Three-week-old female mice were maintained in standard or enriched housing up to and through ovariectomy and behavioral testing at 5, 17, or 22 months of age.
An increased SOD activity in the right hippocampus of HI group maintained in standard environment was found, this effect was reversed in HI enriched group.
After that, 3-D cell culture was maintained in standard conditions.
The cells were maintained in standard culture conditions with 10% fetal calf serum and penicillin/streptomycin at 37°C.
Ammonia, nitrite and nitrate were monitored using a commercial testing kit (Fish Farming Test Kit, Model FF-1A; HACH Co., Loveland, CO, USA) and maintained in standard ranges.
The protocol followed all National Institute of Animal Health (NIAH) rules: during the experiment animals were maintained in standard conditions of 12 hours light-dark cycle, food and water ad libitum, 24±1°C, humidity above 55%.
Post-operative mice were maintained in standard animal house conditions.
The Tg(fli1 EGFP y1 transgenic line [10] was maintained in standard conditions as previously described [19].
