Mice were kept in the animal facilities of the medical faculty of the University of Rostock and maintained in specified pathogen-free conditions.
For xenograft passage (passages used here from P4 to P17), tumour fragments were subcutaneously grafted into 5-week-old athymic nude female mice (Harlan, Winkelmann, Germany) bred and maintained in specified pathogen-free conditions (protocol approval n°P2.VDM.026.07, local ethical committee on animal experiments, CREEA René Descartes, Paris, France).
For xenograft passage, tumour fragments were subcutaneously grafted in the interscapular region into 5-week-old athymic nude female mice (Harlan, Winkelmann, Germany) bred and maintained in specified pathogen-free conditions (protocol approval no P2.VDM.026.07, local ethical committee on animal experiments, CREEA René Descartes, Paris, France).
ad libitum and maintained in a specified pathogen-free state (SPF) under a strict light cycle (lights on at 06:00 h and off at 18 00 h).
Data entry and storage, both paper and electronic will be managed and maintained as specified in the Human Research Ethics Committee HRECC) approvals for a period of not less than 15 years.
EF43.fgf-4, which served as control cells unless otherwise specified, were maintained in DMEM containing high glucose and 10% FBS.
Phenotypes and elements of the genome-scale model to be maintained in the reduced model were specified as follows.
Glioblastoma cell lines were obtained from the German tissue bank (DSMZ) unless otherwise specified and maintained in RPMI (DBTRG), DMEM (GAMG, SNB19, U87, U118, CCF, LN229), MEM (8MG, T87G), DMEM/F12 (1 : 1) (MO59K, Mor9K) or MEM/RPMI 1 : 1 (42MG) supplemented with 10% foetal bovine serum (All purchased from Invitrogen, Paisley, UK) and 2 mmol/l L-glutamine (Sigma-Aldrich, Dorset, UK).
The paper analyses the structural, semantic and logic properties of the demonstration that an embedded computer-based system is adequately specified, designed and maintained in operation.
To isolate the kinases detected in the in-gel assay, GST-bZIP63 was immobilized on GST-Sepharose and plant protein extracts made from root cultures or root-derived cell suspension (as the latter is maintained in the presence of sucrose, please specify which of these sample is shown in Figure 2) were subjected to affinity binding followed by NaCl elution.
Where specified, GV oocytes were maintained in GV arrest by supplementing the medium with 300 µM dbcAMP, which completely and reversibly maintains arrest of mouse GV oocytes [11].
