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A run is labeled as ecologically stable if crossfeeding is maintained in short ecological simulations (25 000 time steps, no mutations).
In addition, breast cancer tissue maintained in short term culture was previously shown as a potential model to study the activity of drugs (i.e. paclitaxel) and hormones (i.e. estrogen and calcitriol) [ 19- 22].
It seems highly likely that regulation of DIO2 by TSHβ underlies seasonal cyclicity in both the reproductive and metabolic axes, indeed a recent study in Siberian hamsters maintained in short days has demonstrated that intracerebroventricular infusion of TSHβ increases body weight in addition to promoting testicular recrudescence (Klosen et al., 2013).
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The islets were maintained in short-term tissue culture (<24 h) in RPMI-1640 supplemented with 10% calf serum, 100 U/ml penicillin and 10 µg/ml streptomycin (all from Life Technologies, Täby, Sweden).
Primary cultures of normal human oral keratinocytes were established and maintained in short-term culture as described [ 30- 32].
1-Naphthol was selectively toxic to human colorectal tumours compared to corresponding normal colonic tissue removed at surgery and maintained in short-term organ culture.
The topography of stimulus-specific components seems to depend on the particular feature that is to be attended or maintained in short-term memory.
Primary cultures of human airway epithelial cells were obtained by enzymatic digestion of bronchial samples from lung transplants and maintained in short-term culture as described [ 33, 34].
Control and tumour tissue was excised at varying periods and maintained in short-term organ culture in the presence of 3H- or 14C-fucose.
Histamine-producing enterochromaffin-like cells, which can be partially purified and maintained in short-term culture, are regulated by voltage-dependent calcium entry but are not believed to be electrically active, as they lack voltage-gated sodium currents (Bufler et al., 1998).
For some experiments (see Figs 1, 2, 3, and 6), the islets were dissociated into single cells, plated on to plastic Petri dishes and maintained in short-term (up to 48 h) tissue culture as previously reported [ 17], whereas intact islets were used for the remaining experiments.
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