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Chemoresistant cell lines were maintained in selective medium containing the highest tolerable concentrations of carboplatin (2.0 µg/ml for SKOV3-CR, 1.0 µg/ml for A2780-CR, and 0.5 µg/ml for OVCAR3-CR) [6].
Cells were maintained in selective media containing 100 μg/ml hygromycin and 15 μg/ml blasticidin.
CHOR1.1-ANTXR1-HA and CHOR1.1-ANTXR2-HA were maintained in selective media.
T. vaginalis strain C1 was then transfected by electroporation as previously described [ 12] and maintained in selective media containing G418.
A549-pAcGFP1-Mem cells were maintained in selective cell culture medium (DMEM with 10% FBS) containing 200 μg mL−1 G418.
The stable clones DAOY V11 (empty vector transfected) and DAOY M2.1 (MYC vector transfected) were maintained in selective medium in the presence of 500 mg/ml G418 [ 20].
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The cells were always maintained in the selective media containing 2 µg/ml puromycin (InvivoGen).
The G418-resistant clones were pooled, amplified, and maintained in the selective growth media.
All stable cell lines were maintained in the appropriate selective antibiotics.
Once formed, high resistance is maintained in absence of selective pressure resulting in MRSA with homogeneous oxacillin resistance.
These results indicate that ura- selection is required for the transformant to show laccase activity, which is maintained in ura- selective cultured media.
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