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The observation that PCN expression is maintained in secondary vascular tissues but not primary vasculature of leaves could reflect differences among species, but more likely reflects differences among these tissue types.
Metastatic gastric and liver tumours were also found to be CD133-positive indicating that expression is maintained in secondary tumours.
It is believed that memory CD8+ T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation.
The figure legend is not clear, and the text states that PD-1 expression is maintained in secondary hosts that had not been exposed to PIT, but lacks sufficient detail.
In fact, even though Treg numbers were adequately maintained in secondary lymphoid organs other than the transplant, the inability to maintain them within MHC-II KO lymph node transplants resulted in the local activation of T cells, which in the long-term resulted in the rejection of the MHC-II KO transplants.
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Interestingly, PCN expression is maintained in older secondary xylem, and appears to be associated with rays (Figure 3).
(C and D ) X-gal staining was maintained in most secondary neurospheres upon passaging of E12.5 Imp1 β-geo/+ dorsal telencephalon-derived primary neurospheres (C ).
The dissociated primary spheres were maintained in a T25 flask for secondary sphere formation.
The normal maternal imprints of SNRPN and KCNQ1OT1 in the GV and MI oocytes were stably maintained in vitro up to the secondary oocyte stage (Geuns et al., 2003, 2007b; Table III).
Recombinant chromosomes Ab and Ba (the superscripts indicate B genome alleles in an A genome background, and vice versa) could then be transmitted to gametes and maintained in the population, leading to secondary diploids with genome constitutions such as AbA, AbB, ABa and BBa and eventually to triploids of constitution AbAA, AbAB, etc.
Significant phenotypic differentiation between genetically homogenous (in terms of neutral variation) populations from Cumaná region suggests that divergent mate preferences documented in earlier work [ 12] can maintain variation in secondary sexual traits even in the face of considerable gene flow.
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