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We have previously shown that human pancreatic islets can be maintained in rotating wall vessels for up to one week without a significant loss of viability [ 16].
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Cells were seeded dynamically onto biodegradable scaffolds, which then were maintained in a rotating bioreactor for 6 to 8 weeks.
Alternatively, 985 mg of dry weight samples were suspended in 2.94 mL Na citrate buffer including enzymes, placed in 6-mL glass vials containing two 5-mm glass beads and incubated at 47 °C on a rotating wheel (vials maintained in horizontal orientation) at ~2 rpm.
The growth uniformity in the layers was maintained by rotating the substrate at 20 rpm during deposition.
The needle is maintained in place for 20 minutes, and rotated for one minute every five minutes (i.e. four times per session).
The revival, which launched last year with Neil Patrick Harris and walked away with four Tony Awards, has maintained a rotating roster of stars since Harris left, bringing in actors including Andrew Rannells, Michael C. Hall, original star John Cameron Mitchell and Criss for stints in the show.
Between sampling the serum bottles were maintained in the dark at 20°C on a rotating shaker.
A rotating detachment of three Hercules was subsequently maintained in the Middle East to support the ongoing Australian contribution to the War in Afghanistan, as well as the forces stationed in Iraq.
FreeStyle 293-F suspension cell culture was maintained in 125 500 mL Erlenmeyer flasks containing 30 120 mL of FreeStyle medium in a 37°C incubator with a humidified atmosphere of 8% CO2, on an orbital shaker platform rotating at 135 rpm.
Cell suspensions were maintained in darkness at 25 ± 2°C on semisolid media, in 125 ml Erlenmeyer flasks (60 rpm), 1 L nippled flasks on a clinostat (1 rpm simulating 2 × 10-4 × g, but with significant convective mixing of the gaseous and liquid environment), 100 ml high-aspect rotating vessels (HARV 12.5 cm dia)., and rotating cylindrical culture vessels (RCCV 7.5 cm dia).
Waves in rotating media.
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