Sentence examples for maintained in regular from inspiring English sources

Cells were then switched to low-glucose growth medium (DMEM containing 1 g/l glucose plus 10% FBS) or maintained in regular growth medium for additional 48 h.

Primary murine hepatocytes obtained from WT mice were isolated24,25,31 and cultured and maintained in regular DMEM growth media (Sigma, #D5796) containing 10% FBS.

Next, to examine how TRIM28 regulates TRIM24 transcriptional program, we performed triplicate microarray experiments profiling LNCaP cells maintained in regular medium with pLKO control, TRIM24, TRIM28 knockdown, or TRIM28 knockdown with TRIM24 rescue.

Infected cells were then split and maintained in regular medium until use.

The sorted T cells were maintained in regular T cell culture medium and restimulated with 1ug/ml PHA weekly in the presence of 50 Gy irradiated allogenetic PBMCs and 10 IU/ml of IL2.

In addition, consistent with the previous findings (Fig. 4B), parallel co-cultures of myoblastsFLPe and myoblastsGS.Luc maintained in regular growth medium did not show a time-dependent rise in luciferase expression despite the increase in cell numbers due to mitosis (Fig. 5B, solid bars in right panels).

In order to visualize astrocytes for patch-clamp recording, the slices were first incubated in warm ACSF (30 °C) containing 1 μM sulphorhodamin-101 (SR101) for 20 min and then transferred and maintained in the regular ACSF at room temperature until placed in the recording chamber.

The androgen-dependent LNCaP cell line was obtained from American Type Culture Collection (Rockville, MD) and was routinely maintained in a regular medium: phenol red-positive F-12 medium (Gibco) with 10% FBS (Biowest) at 37°C in 5% CO2.

Cells maintained in the regular culture medium served as controls.

The internodes were maintained in a regular refrigerator at a ~4°C during few minutes (0 h treatment), 24 and 60 h after field harvested.

Negative Control: Cells maintained in the regular cell culture medium for the entire experiment (DMEM with 20 % FBS, 1 % Pen-Strept, 1 % non-essential amino acids).