Sentence examples for maintained in monolayer from inspiring English sources

Acute insulin secretory responses to glucose and amino acids were compared between microencapsulated cells and non-encapsulated cells maintained in monolayer culture.

Human Sox9 mRNA levels were constantly maintained in monolayer cell culture conditions over a period of 21 days, while these were upregulated when chondrocytes were cultured on HYAFF-11 and HYAFF-11S.

All cell lines are maintained in monolayer culture conditions described previously [26].

Human breast carcinoma MDA-MB-231 and human fibrosarcoma HT-1080 cells were routinely maintained in monolayer culture in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS; Invitrogen).

The human fibroblast cells HFL1 CCL-1533) derived from normal lung tissue [69], adenocarcinoma A549 cells, NIH 3T3 and HEK 293 were purchased from the American Type Culture Collection (Manassas, VA) and maintained in monolayer culture in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS).

Spodoptera frugiperda (Sf9; GibcoBRL, Grand Island, NY/CRL 1711, ATCC) insect cells were maintained in monolayer and suspension cultures at 28°C using serum-free Insect-XPRESS culture medium (Cambrex, Walkersville, MD) or HyQ®SFX-Insect medium (HyClone Inc, Logan, UT) without antibiotics.

Explant cells from cNSCs (OVC.EX lines) could be maintained in monolayers up to and beyond P6 while retaining SOX2+ expression (Fig. 5A, B) and glia and neuronal potential.

First, the expression of type I and type III procollagen was evaluated sequentially for 1 week in primary cultured human articular chondrocytes maintained in monolayers.

The cells were maintained in monolayers in tissue culture flasks (75 cm, Falcon, Becton Dickinson-Gambil, Heidelberg, Germany) in RPMI 1640 medium (GIBCO, Karlsruhe, Germany), supplemented with 10% foetal bovine serum (GIBCO), penicillin and streptomycin (GIBCO).

Chondrocytes were isolated from human septal cartilage of patients from 15 to 60 year old and maintained in primary monolayer culture for 14 days.

T47D cells were maintained in a monolayer culture in RPM1 containing 1% Glutamax 1 and10% FBS.