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Fibroblast cultures were maintained in modified Eagle's medium (EMEM) or Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS) (Gibco BRL, Grand Island, NY), 1% vitamin solutions, and 2 mM L-glutamine.
SW480, HCT116, and CCD-841-CoN were propagated in Dulbecco's modified Eagle's medium (DMEM), and HT29 were maintained in modified RMPI-1640 medium, both supplemented with 10%% fetal bovine serum, 100 μg/mL penicillin, 100 μg/mL streptomycin.
Infected cultures were maintained in modified M199 medium for 24 h before analysis.
A498 cells were maintained in modified Eagle's medium (MEM) supplemented with 10% FBS, 1× sodium pyruvate and 1× l-glutamine.
Following in vitro selection, they were maintained in modified IMEM-phenol red free with 5% charcoal-stripped FBS.
Stage VI oocytes were collected from female Xenopus laevis and maintained in modified Barth's saline supplemented with 25 µg/ml Gentamicin (Sigma).
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Cells were maintained in these modified composition media for a total of 9 days, including a splitting process at day 4 of incubation.
MDCK and 293T cells were maintained in Dulbecco׳s modified Eagle׳s medium with 10% (v/ v) fetal bovine serum (FBS; Invitrogen).
LNCaP cells from ATCC were maintained in a modified RPMI1640 medium supplemented with 25 mM HEPES buffer, 1 mM Na.pyruvate, 1 mM glutamine, 4.5% glucose and 10% FBS.
The human gastric adenocarcinoma cell lines AGS were obtained from ATCC and maintained in Dulbecco modified essential medium (DMEM), supplemented with 10% FBS.
SK-MEL-1 was maintained in alpha modified Eagle's Minimal Essential Media (MEM) supplemented with 10% fetal bovine serum (FBS), and Daudi and LNCaP were maintained in RPMI-1640 Media supplemented with 10% FBS.
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