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In order to ensure the normal operation of WMSNs, the energy consumption of communication must be maintained in minimum level.
Daoy cells were propagated in Dulbecco's modified Eagle's medium (DMEM), D283 cells were maintained in minimum essential medium (MEM), both supplemented with 10%% fetal bovine serum, 100 units/mL penicillin G sodium and 100 μg/mL streptomycin (HyClone).
Cells were maintained in Minimum Essential Medium as described previously [17].
Rabbit kidney (RK13) cells were maintained in minimum essential medium (MEM, Mediatech Inc).
The human choriocarcinoma-derived placental JEG3 cell line was maintained in Minimum Essential Medium (SIGMA) supplemented with 10% fetal bovine serum.
SF126 was maintained in Minimum Essential Medium (MEM) (Gibco) with 10% FBS, 100 U/ml penicillin and 0.1 mg/ml streptomycin.
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In addition, samples were maintained in-vivo for a minimum of 8 weeks.
HeLa cells and 293T cells were maintained in Dulbecco minimum essential medium (DMEM; GIBCO, Carlsbad, USA) at 37°C in 5% CO2.
The cell cultures were maintained in Eagle Minimum Essential Medium with Earle's Balanced Salt Solution (EMEM, ATCC, Manassas, VA) and subcultured with Trypsin-EDTA (Invitrogen, Carlsbad, CA).
Murine embryonic stem cells (CGR8) and those expressing MLC2v-GFP coupled to the cardiac α-actin promoter [81] were maintained in Glasgow Minimum Essential Medium (BioWhittaker-Cambrex) [74], [82].
MCF7 human breast adenocarcinoma cells were maintained in Eagles Minimum Essential Medium supplemented with 10% (v/v) FCS.
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