Sentence examples for maintained in minimal from inspiring English sources

The cells were maintained in minimal essential medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum, 1% non-essential amino acids and an antibiotic-antimycotic mixture.

The cells were maintained in Minimal Essential Medium supplemented with 10% FBS, penicillin (100 U/ml), and streptomycin (100 μg/ml) in a humidified atmosphere of 50 μg/ml CO2 at 37 °C.

Rat basophilic leukemia (RBL) cells (RBL-2H3; ATCC number CRL-2256) were maintained in minimal essential medium (MEM) supplemented with 10%% FBS, 1 % Pen-Strep, and 1 % l-glutamine (L-glut).

Human liver hepatocellular carcinoma, Hep G2 cells were maintained in minimal essential medium (MEM) containing 3,7 g/L sodium bicarbonate, 4,5 g/L D-glucose, 4,7 g/L 4- 2-hydroxyethyl -1-piperazineethanesulfonic acid (HEPES), 4- 2-hydroxyethyl -1-piperazineethanesulfonic U/ml penicillin, 100 U/ml streptomycin and 10% (v/v) foetal bovine serum.

All signalling mutants, their isogenic recipients, and the actin-GFP strain were grown and maintained in minimal medium (MM, Ronen et al., 2007) with respective supplementation (Bussink and Osmani, 1999; Shimizu and Keller, 2001; Guest et al., 2004, Ronen et al., 2007; Taheri-Talesh et al., 2008).

AH109 strains harboring pGBKT7 plasmids were maintained in minimal SD media with tryptophan dropout supplement (SD/-Trp) while Y187 strains harboring pGADT7 plasmids were maintained in minimal SD media with leucine dropout supplement (SD/-Leu).

HEC-1-B (ATCC HTB 113) human endometrial adenocarcinoma cell line were maintained in Eagle minimal essential medium supplemented with 10% FBS at 37°C, 5% CO2.

These studies used CHO WTP4 cells, which are not killed by LF, and the cytotoxic fusion protein FP59 [27], [27], in which the N-terminus of LF is fused to the ADP-ribosylating domain of Pseudomonas exotoxin A. CHO cells were maintained in alpha minimal essential medium supplemented with 5% fetal bovine serum, 2 mM glutamine, 50 µg of gentamicin/ml, and 25 mM HEPES.

Cells were cultured and maintained in supplemented minimal essential medium (MEM) as previously described (Jung et al, 1999).

Cultures were maintained in MEM (minimal essential medium; Invitrogen), Earle's salts supplemented to 10% with FBS (foetal bovine serum; Atlanta Biologicals) and 10 μg/ml gentamycin (Sigma).

Salmonella enterica serovar Typhimurium strain SL1344 was maintained in basic minimal medium (BMM) (10) with 40% glycerol or in tryptone soy broth (TSB) with 40% glycerol, stored at −80°C.