Sentence examples for maintained in media from inspiring English sources

When T lymphocytes were incubated with PEG-HCCs for 30 min to allow internalization, washed, and maintained in media at 37 °C, both external and internal levels of PEG-HCCs decreased over 6 h (Fig. 2d and Supplementary Fig. S8), demonstrating that the nanoparticles do not accumulate inside the T cells over time.

MCF 10A cells were maintained in media containing horse serum as previously described [99].

The transfected cells were maintained in media containing 500 ug/ml Geneticin.

However, the inhibitory effect was lost by day 5 in cells maintained in media without peptide.

A subset of unmodified 3T3-L1 cells were expanded, induced, and maintained in media containing the chemical uncoupler FCCP.

After two weeks of G418 selection, transfected cells were maintained in media containing 1 mg/ml G418.

After a 24-h recovery in complete media without virus, polyclonal stable cell lines were selected and maintained in media containing 5 µg/ml puromycin.

After isolation, colonies were maintained in media lacking neomycin and maintenance of Venus fluorescence was assessed by fluorescence-activated cell sorting (FACS) (Figure 6B).

A mixed population of puromycin resistant cells from clone RHS3979-98487921 were maintained in media containing puromycin and used in all the experiments.

For all experiment periods, cells were maintained in osteogenic media, and media was changed every three days.

Ba/F3 cells were maintained in RPMI1640 media supplemented with 10% fetal bovine serum (Gemini) and 10% WEHI-conditioned media.