To determine whether human IL-4R expression is maintained in xenografts, we examined mRNA expression by real-time RT-PCR for IL-4R α, IL-13R α1, and IL-2R γC.
In the presence of early Sxl protein product (SXL), female-specific splicing of Sxl pre-mRNA is maintained in females through autoregulation, and exon 3 of Sxl pre-mRNA is removed after splicing [8].
M14 cell clones stably expressing an ecdysone-inducible MYC antisense mRNA were maintained in RPMI-1640, aboveove, in the presence of both G418 (GIBCO) and Zeocyn (Invitrogen) [16].
Some of the mRNAs are maintained in a translationally repressed state and may be transported to a specific cytoplasmic location where translation is activated [ 46].
The expression of cartilage- and disc-specific mRNAs was maintained in hydrogels in vitro and in vivo, demonstrating the maintenance of a stable specific cellular phenotype, compared to monolayer cells.
In WSN, mRNA levels peaked at 9 hours and then steadily decreased (Figure 2C, lanes 1-6); however, mRNA levels were maintained in both HK and SW (Figure 2C, lanes 7-12 and 13-18).
How mRNA translation is maintained in this circumstance is unclear.
After hatching, pc/glis3 mRNA expression was maintained in the renal tube structures, the tubule, and the duct (Fig. 2H, I, L, M, N).
qRT-PCR analysis also confirmed that elevated E-cadherin mRNA levels were maintained in the primary tumors derived from the miR-141-200c transduced 4TO7 cells (Figure 8C).
All together, these results suggest that RXR-γ and RAR-β steady-state mRNA levels are maintained in normal conditions and regulated by axonal contact mimickers throughout different mechanisms.
