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M. tuberculosis strain H37Rv stably expressing mCherry was maintained in logarithmic phase at all times in 7H9 medium (BD Difco Middlebrook) containing 50 µg/ml hygromycin in a BSL-3 laboratory.
Cells were maintained in logarithmic growth as described below.
Cells were maintained in logarithmic growth phase for all experiments.
The cells were maintained in logarithmic growth by passage every 3-4 days.
Using this scheme, cells were maintained in logarithmic growth throughout the time course.
Before the start of each experiment, the YAC-1 cells were maintained in logarithmic growth in T25 culture flasks.
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Cell lines were cultured as described above and passaged on consecutive days to maintain in logarithmic growth phase immediately prior to transfection.
For measuring drug response in the AZCL panel, cell lines were maintained in the logarithmic phase of growth.
The cells were maintained in the logarithmic phase of growth and were subcultured at 2 3 day intervals.
They were maintained in the logarithmic phase of growth in RPMI-1640 medium supplemented with 2 mM L-glutamine, 100 IU/ml penicillin, 100 μg/ml streptomycin, and 10 % FCS at 37 °C under humidified air with 5%% CO2.
All cell lines were maintained in a state of logarithmic growth at 37 °C in humidified air with 5% CO2, as described previously.18, 24, 25, 27 For RNA analysis, cultures were harvested at the same final cell density (5 × 105 per ml), and processed immediately.
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