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Since that time the strain has been widely disseminated and maintained in laboratories around the world.
Although I. ricinus ticks can be routinely maintained in laboratories, SIB TBEV is normally associated with transmission by I. persulcatus, which is not as readily available for laboratory experiments.
The most widespread and ecologically diverse group is also the best represented in the laboratory: six P. maniculatus subspecies are maintained in laboratories across the United States.
Notably, as many as 87.6% of the newly discovered SNPs are novel, compared to SNPs in dbSNP (NCBI, release 125) [ 15] that were mainly from mosquito colonies maintained in laboratories.
SiHa, HeLa (S), and HeLa (K) were grown and maintained in laboratories of Dr. Ponglikitmongkol M, Mahidol University, Dr. Gutkind JS, NIH, USA and Dr. Ruxrungthum K, Chulalongkorn University, respectively.
Its application is most utilized to characterize unculturable organisms (an estimated 99% of microbes cannot be cultivated in a laboratory environment [ 1]), but it has also made it possible to sequence genomes without the problems that are associated with cultures maintained in laboratories [ 2].
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The research groups carried out their studies on four different kinds of Drosophila cells maintained in laboratory cell cultures, not all of which had been extensively explored before.
O. dioica can be maintained in laboratory culture, and is of growing interest as a model organism because of its phylogenetic position within the closest sister group to vertebrates.
The Ae. aegypti mosquito strain UGAL was maintained in laboratory culture as has been previously described by Hays and Raikhel [33].
We find that morphological (bill size and plumage coloration) and life history (reproductive effort) differences observed between wild populations were maintained in laboratory raised individuals suggesting genetic divergence of fitness related traits.
A. vulgare lineages infected with wVulC or wVulM are maintained in laboratory for decades showing that the symbiotic transmission and feminizing phenotypes of these two Wolbachia strains persist through generations (Bouchon et al., unpublished data).
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