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Rats were maintained in individually ventilated cages (Tecniplast) under standardized conditions (at a temperature of 21±2°C, a humidity of 65±5% and with an artificial 12 h light/dark cycle) with free access to standard chow (0.25% sodium; SSNIFF) and drinking water ad libitum.
All animals were maintained in individually ventilated cages.
Mice were maintained in individually ventilated cages (IVC) (Allentown Inc., NJ, United States).
Rats were maintained in individually ventilated cages (4 per cage) in the Animal Holding Facility (AHF) at the International Medical University (IMU) after their arrival.
Severe combined immune-deficient (SCID) mice and Swiss Webster mice were obtained from Taconic Farms and were maintained in individually ventilated cages.
BALB/c mice were maintained in individually ventilated cages (IVC) (Rair Isosystem, Laboratory Product Inc., USA) and fed with standard chow and water ad libitum.
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Animals were maintained in individually-ventilated cages on a 12 h light-dark cycle, with free access to food and water.
Mice were specific pathogen free and maintained in sterile conditions in individually ventilated cages by the Biological Services Faculty (BSF), University of Manchester, UK.
The bivalves were individually maintained in rearing cups in which salinity was decreased either gradually (over 1 11 h to 12 salinities ranging from 0 to ca. 34 ppt) or rapidly (directly to 21 salinities) at 12 and 24 °C.
Paired explants were individually maintained in explant culture.
Leptodora were individually maintained in 50 ml vials filled with synthetic freshwater at 21°C with 24 h light photoperiod.
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