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All strains were maintained in heart infusion agar slants (HIA, Difco), and prior to assay, the cells were grown overnight at 37°C in brain heart infusion (BHI, Difco).
The strains were maintained in heart infusion agar slants (HIA, Difco), and prior to assay, the cells were grown overnight at 37°C in brain heart infusion (BHI, Difco).
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Its expression is maintained in the heart throughout prenatal and postnatal life.
The Vibrio strains were maintained in Brain Heart Infusion (BHI) medium, supplemented with 3% NaCl.
The bacterial isolate was maintained in Brain Heart Infusion (BHI) agar (Pronadisa, Spain) slants at 4°C.
Pure liquid cultures of P. aeruginosa and I. limosus were made in TSB whereas D. pigrum was maintained in brain heart infusion broth (BHI, Liofilchem, Italy).
C. pseudotuberculosis biovar ovis strain 1002, isolated from a goat, was maintained in brain heart infusion broth (BHI; HiMedia Laboratories Pvt. Ltd., Mumbai, India) at 37°C.
These strains were maintained in brain-heart-infusion broth (BHI – HiMedia Laboratories Pvt. Ltda, India) at 37°C, under rotation.
E. coli strains were routinely grown in Luria-Bertani (LB) broth or on LB agar plates, and P. aeruginosa strains were maintained in brain-heart infusion (BHI) broth or on BHI agar plates.
Furthermore, unlike WT animals, adrenergic sensitivity and reserve are maintained in KI hearts treated with ISO, consistent with a sustained permissive physiological response to adrenergic stimulation.
Although electrical conduction and Ca2+ handling in the adult heart are well understood, little is known about how these functional characteristics are established and maintained in the embryonic heart, and how they impact cardiac morphogenesis and differentiation.
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