The neurons were maintained in growth media consisting of MEM supplemented with B27, glutamine (all from Invitrogen), glucose, transferrin (Calbiochem), fetal bovine serum and Ara-C (both from Sigma) for a week before co-culture with iNs.
The ESCs selectively attached to the micropatterned features and expressed markers associated with a pluripotent phenotype, such as E-cadherin and alkaline phosphatase, when maintained in growth medium containing leukemia inhibitory factor.
When log phase cells of wild-type E. coli K-12 were maintained in growth medium after X irradiation, they became progressively more resistant to a subsequent exposure to UV or X radiation.
The murine MΦ cell line RAW264.7 was purchased from ATCC (Manassas, VA) and maintained in growth media (DMEM Life Technologiess, Carlsbad, CA) supplemented with 10% FBS and 1% penicillin/streptomycin).
3T3-L1 preadipocytes were maintained in growth medium (Preadipocytes) or induced for adipocyte differentiation in the absence (Control) or presence of 1 or rosiglitazone for 8 days.
3T3-L1 Preadipocytes were maintained in growth medium (Undifferentiated), or induced for adipocyte differentiation in the absence (Differentiated control) or presence of various concentrations of 1 or rosiglitazone.
Control cells were maintained in growth medium containing 0.1%DMSO.
Six hours post infection, the virus was removed by washing and the cells were maintained in growth media.
Subconfluent N2a cells were maintained in growth media (1 1 DMEM OptiMEM-I mixture containing 5% FBS anDMEM OptiMEM-Imixture
The stable cell line 2008-Ets 1 [27]was maintained in growth medium as described with the addition of 200 ng/ml of the selective antibiotic Zeocin.
Rabbit aortic smooth muscle cells (VSMC) were obtained from a previously established selection-immortalized line, as reported previously [26] and maintained in growth medium (F12+10% fetal bovine serum + streptomycin 100 µM + penicillin 100 U/mL).
