Sentence examples for maintained in glucose from inspiring English sources

The labeled cells were maintained in glucose free-DMEM for 2 h at the indicated glucose concentrations and then incubated with 0.4% 1-butanol for 5 min. Next, cells were harvested into 0.8 ml of methanol and 1 M NaCl (1∶1) and then mixed with 0.4 ml of chloroform.

Cardiomyocytes were maintained in glucose and serum-free DMEM (Gibco, Carlsbad, CA, USA) during hypoxic tenure.

After incubation for 12 h at 37°C in 5% CO2 atmosphere, cells were either maintained in glucose medium or switched to galactose medium for 12 h.

To test growth capability, transformed cells were maintained in glucose medium for 24 h and then harvested and seeded at a constant number of cells (30 × 10) in 60-mm dishes in glucose, galactose or GPM.

To determine the steady-state levels of mt-tRNALeu UUR) WT, RNI64 and transiently trasformt-tRNALeu UURre maintained in glucose medium for 12 h and then collected for RNA extraction.

To test the rate of apoptosis transformed cells were maintained in glucose medium for 24 h and then harvested and seeded at 0.5 1 × 10 in glucose, galactose or GPM for 24 h.

Compared with cells maintained in glucose-containing medium, results show that glutamate kills significantly more neurons when the cultures are glucose deprived at every time point tested).

Our results complement those obtained by Krautz-Peterson et al. [ 5] since they showed that RNA interference (RNAi) of both SGTP1 and SGTP4 more strongly affected schistosomula when they were maintained in glucose-poor medium than in glucose-rich medium.

For 1-day galactose experiments, cells were maintained in glucose-supplemented media for 4 to 5 days prior to replacing with fresh glucose or galactose media, thereby minimizing differences in total culture duration.

Interestingly, nuclear translocation of NF-κB p65 by high glucose and Cx43-silencing was maintained in normal glucose.

Indeed, data shows that levels of HIF-1α were downregulated under hypoxia in cells treated with high glucose over a period of 10 days, as compared to cells maintained in basal glucose concentration (Figure 1A).