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Different approaches are used ranging from in situ perfusion (intestinal loop) models to models in which part of the gut epithelium is excised from animals and maintained in, for example, Ussing chambers for a limited period of time.
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In addition, samples were maintained in-vivo for a minimum of 8 weeks.
Transwell co-cultures were maintained in GM for 7 days and then harvested for analyses.
Uninfected DCs maintained in culture for 5 days served as controls for baseline levels of GSH.
For experimental use, cells were thawed and maintained in culture for no longer than 10 passages.
Immunocytochemistry experiments for localization of GAD65 were performed using hippocampal neurons maintained in culture for 10 days.
Organoids were maintained in culture for ~6 9 days before being propagated into additional wells or used for experimental procedures.
After that, the culture was maintained in freezer for further use [11].
Leaves were maintained in darkness for 30 min before taking the data on Chl fluorescence.
Properties of BMs are maintained in homeostasis for most adult tissues.
Seven arrived at age 3 months and were maintained in house for approximately 9 months or to 1 year of age, and two HL pigs arrived at age 11 months and were maintained in house for 1 month.
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CEO of Professional Science Editing for Scientists @ prosciediting.com